Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Structural Maintenance of Chromosomes Protein 3 (also known as SMC3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human SMC3 antibody. SMC3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human SMC3 Antibody is added and binds to SMC3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SMC3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human SMC3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm